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Volume 43 Number 5, September/October 2006
Pages 713 — 722


Abstract - Migration and proliferation of retinal pigment epithelium on extracellular matrix ligands

Hao Wang, MS, MD; Yancy Van Patten, MS; Ilene K. Sugino, MA; Marco A. Zarbin, MD, PhD*

Institute of Opthalmology and Visual Science, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ
Abstract — We compared aged adult and fetal retinal pigment epithelium (RPE) migration and proliferation in cell culture. Aged adult RPE resurfaced on dishes coated with human or mouse laminin, human or rat collagen I, human or mouse collagen IV, or human fibronectin. Resurfacing was best on bovine corneal endothelial cell extracellular matrix. Resurfacing was worst on mouse laminin. Fetal RPE resurfacing was robust on all surfaces studied except human and mouse laminin. In general, fetal RPE resurfacing was much greater than that seen with aged RPE on comparable surfaces. The degree of cell proliferation (labeled by Ki-67) was much greater in fetal than adult RPE cells.
Key words: age-related macular degeneration, aged retinal pigment epithelium, bovine corneal endothelial cell extracellular matrix, cell migration, cell proliferation, choroidal neovasculari-zation, extracellular matrix, fetal retinal pigment epithelium, retinal pigment epithelium resurfacing, wound healing.

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