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Volume 45 Number 6, 2008
   Pages 931 — 938

Abstract - Effects of low-level laser therapy on mast cell number and degranulation in third-degree burns of rats

Mohammad Bayat, PhD;1-2* Mohammad Mehdi Vasheghani, MSc;3 Naser Razavie, PhD;3 Mohammad Reza Jalili, MD2

1Cellular and Molecular Biology Research Center, 2Anatomy Department, Medical Faculty, and 3Paramedical Faculty, Shahid Beheshti University, M.C., Tehran, Iran

Abstract — Mast cells have been shown to participate in the wound healing process. We investigated the effects of low-level laser therapy (LLLT) on mast cell number in the inflammation, proliferation, and remodeling phases of the wound healing process of experimental burns. Sixty rats subjected to third-degree burns were divided into four groups: two laser-treated, one control, and one nitrofurazone-treated group. In the two laser-treated groups, burned areas received LLLT with a helium-neon laser at energy densities of 38.2 J/cm2 and 76.4 J/cm2, respectively. The effects on mast cell number and degranulation were assessed 7, 16, and 30 days postburn (inflammation, proliferation, and remodeling phases of wound healing, respectively). Intact and degranulated mast cells were counted. Five rats with no burns were used for baseline studies. On day 7 in the first laser group, the total number of mast cells was significantly higher than in the other groups. On day 16 in the nitrofurazone-treated group, the total number of mast cells was significantly higher than in the control, first laser, and normal groups. LLLT on the experimental third-degree burns significantly increased the total number of mast cells during the inflammation phase of wound healing; also, topical application of 0.2% nitrofurazone ointment on the same burns significantly increased the total number of mast cells during the proliferation phase of burn healing.

Key words: burn, cell count, degranulation, helium-neon laser, histology, low-level laser therapy, mast cell, morphometry, rat, wound healing.


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